เบซิล นักสืบหนูผู้พิทักษ์

Barrie Ingham

Basil of Baker Street / Bartholomew (voice)

Vincent Price

Professor Ratigan (voice)

Val Bettin

Dr. David Q. Dawson / Thug Guard (voice)

Susanne Pollatschek

Olivia Flaversham (voice)

Candy Candido

Fidget (voice)

Diana Chesney

Mrs. Judson (voice)

Eve Brenner

The Mouse Queen (voice)

Alan Young

Mr. Flaversham (voice)

Basil Rathbone

Sherlock Holmes (voice)

Ellen Fitzhugh

Bar Maid (voice)

Walker Edmiston

Citizen / Thug Guard (voice)

Wayne Allwine

Thug Guard (voice)

Tony Anselmo

Thug Guard (voice)

แฟรงก์ เวลเกอร์

Toby the Dog / Felicia the Cat (voice) (uncredited)

Laurie Main

Dr. Watson (archive sound)

Shani Wallis

Lady Mouse (voice)